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CAS 72-57-1

Trypan blue

Trypan blue is a bright-blue dye used to check if cells are alive in research labs and to stain eye tissue during cataract surgery. It matters because long-term, high exposures caused cancer in animals and it can irritate eyes and skin [1][2].

Where It Comes From

Research labs, hospitals (ophthalmology), and facilities that make biological stains; found as powders or water solutions [2][3].

How You Are Exposed

Handling powders/solutions, dust or splashes, skin contact, or accidental ingestion; patients may receive tiny, short-term amounts during eye procedures [2][3].

Why It Matters

Animal studies show increased tumors with chronic dosing; human evidence is limited. IARC has evaluated it as a cancer hazard; it can also cause eye/skin irritation with acute contact [1][2].

Who Is at Risk

Lab and manufacturing workers, ophthalmic surgical staff, and people cleaning spills or waste; pregnant workers should minimize exposure as a precaution [1][2].

How to Lower Your Exposure

Wear gloves, goggles, and a lab coat; avoid creating dust; work in a fume hood; use closed/pre-filled systems in the OR; clean spills with wet methods; dispose as hazardous waste per policy [2][3].

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