← All chemicals

CAS 612-82-8

3,3'-Dimethylbenzidine dihydrochloride

This toxic aromatic amine (also called o‑tolidine dihydrochloride) is used to make dyes/pigments and as a lab reagent. It matters because it’s linked to cancer, mainly for exposed workers [1][2].

Where It Comes From

Manufactured for azo dyes/pigments and analytical reagents; can be present in wastes at dye, plastics, or lab facilities [1][3][4].

How You Are Exposed

Breathing dust or contacting skin during manufacturing, lab use, equipment cleaning, or waste handling; less often via air/water near facilities [2][3][4].

Why It Matters

Classified as reasonably anticipated to be a human carcinogen (NTP) and possibly carcinogenic to humans (IARC Group 2B); also irritates skin/eyes [1][2][3].

Who Is at Risk

Workers in dye/pigment production, labs using o‑tolidine reagents, maintenance/waste handlers, and people without proper ventilation/PPE; neighbors of poorly controlled sites [2][3][4].

How to Lower Your Exposure

Use safer substitutes (e.g., EPA‑recommended DPD methods for chlorine testing), closed systems/local exhaust, protective gloves/eyewear, hygiene, and proper hazardous‑waste disposal [3][5].

References

  1. [1]IARC Monographs, Volume 77: Some Aromatic Amines, Organic Dyes and Related Exposures (o‑Tolidine). WHO/IARC, 2000.
  2. [2]NTP, Report on Carcinogens: o‑Tolidine (3,3'-Dimethylbenzidine). U.S. DHHS/NIEHS.
  3. [3]CDC/NIOSH Pocket Guide to Chemical Hazards: o‑Tolidine dihydrochloride. Centers for Disease Control and Prevention.
  4. [4]EPA IRIS: 3,3'-Dimethylbenzidine (o‑Tolidine) Toxicological Review/Summary. U.S. Environmental Protection Agency.
  5. [5]EPA Method 330.5 (DPD colorimetric) for chlorine in water; EPA drinking water methods. U.S. Environmental Protection Agency.

Track your exposure to 3,3'-Dimethylbenzidine dihydrochloride

Pollution Profile maps your lifetime exposure history to EPA-tracked chemicals.

Get early access

We use cookies and analytics to understand how people use Pollution Profile and improve the experience. We never sell your data. Learn more.